Cole-Parmer IPA Surface Wipes - 70% Isopropyl Alcohol Disinfecting Wipes (1200 Canisters, 100 Wipes per Canister) - Ideal for Cleaning Electronics, Medical Equipment & Office Surfaces

Product Description

VACCINE DEVELOPMENT PROCESS Vaccine development starts in the laboratory identifying one or more antigens against a specific pathogen – either a virus or bacteria. 1. Antigens are the components of a pathogen that can elicit an immune response. Attenuated pathogens, weakened to have their virulence disabled, or specific subunits of microorganisms can be safely purposed in vaccine development. 2. Genetic material is extracted from the pathogens in the form of DNA or RNA. RNA is converted to cDNA through a process called Reverse Transcription. 3. qPCR can be used in virus quantification to determine the virus concentration in both the R&D and production stages. 4-5. Amplified DNA from the pathogen is engineered into a plasmid vector and inserted into living cells. 6. Transformed cells are propagated using cell culture techniques that generate multiple copies of the plasmid. 7. Purified plasmids are then extracted from host cells and growth media using filters and other separation techniques. Read more